Author(s)
Nimil Shukla , Akriti Vigya , Prof Vivek Singh
- Manuscript ID: 140231
- Volume: 2
- Issue: 6
- Pages: 28–30
Subject Area: Other
Abstract
Varanasi, one of the oldest living cities, harbors a rich diversity of ethnomedicinal flora. This study (2025-2026) employs an integrated metabolomics approach—combining targeted quantification of bioactive markers with untargeted profiling via LC-MS/MS—to characterize the chemical landscape of Ocimum sanctum, Withania somnifera, and Azadirachta indica sampled from the Gangetic plains of Varanasi. Phytochemical screening revealed a high concentration of polyphenols and alkaloids. Targeted profiling quantified specific markers like Withaferin A and Ursolic acid, while untargeted metabolomics identified 45 novel metabolites previously unreported in this geographical niche. These findings underscore the chemical uniqueness of Varanasi’s medicinal flora influenced by the local edaphic factors.