FORMULATION AND EVALUATION OF HERBAL ANTI-INFLAMMATORY CREAM FROM DELONIX REGIA SEED OIL

Abstract

Inflammation, a vital immune response to harmful stimuli, can become detrimental when prolonged or excessive, contributing to diseases such as arthritis and cardiovascular conditions. While nonsteroidal anti-inflammatory drugs (NSAIDs) offer effective relief, their long-term use is often limited due to significant adverse effects. In pursuit of safer alternatives, plant-derived therapeutics have gained momentum. This study focuses on Delonix regia seed oil, known for its rich phytochemical profile, to develop and evaluate a topical anti-inflammatory cream. The oil was extracted via Soxhlet method and screened for phytochemicals, revealing the presence of flavonoids, steroids, tannins, and volatile oils. Physicochemical analysis confirmed its suitability for topical use, exhibiting optimal viscosity, saponification value, and emollient properties. An oil-in-water (o/w) cream was formulated using standard excipients and evaluated for organoleptic properties, pH, spreadability, irritancy, and stability. The formulation showed excellent skin compatibility, non-greasiness, and stability over time. In vitro anti-inflammatory activity was assessed using the human red blood cell (HRBC) membrane stabilization method. Results demonstrated concentration-dependent membrane protection, with maximum inhibition of hemolysis (33.6%) at 1000 µg/mL, indicating moderate anti-inflammatory activity. While less potent than ibuprofen (85.2% inhibition), the herbal cream displayed promising therapeutic potential with minimal irritancy. The findings support the use of Delonix regia seed oil in topical anti-inflammatory applications, particularly for populations seeking natural, low-risk alternatives. This research contributes to the advancement of herbal formulations as effective, economical, and safer options in managing inflammation-related skin conditions.

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